DNA vs RNA - sites.isdschools.org Differences between DNA and RNA 1. DNA is double stranded, RNA is a single stranded molecule 2. DNA uses the base Thymine, while RNA uses the base Uracil instead 3. DNA has deoxyribose as its sugar, RNA has ribose as its sugar 4. DNA is usually only found INSIDE the nucleus, while RNA is found outside of the nucleus, in the cytoplasm of the cell 5.